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Fig. 1. Lhx5 promotes forebrain development. In this and subsequent figures,
the probes used for whole-mount in situ hybridization are listed in the upper
right corner of each panel. Genotypes or experimental manipulations are
indicated in the lower left corners. Developmental stages are indicated in the
lower right corners. Unless otherwise noted, gastrula stage embryos are
orientated in animal pole view, rostral to the top; post-gastrulation stage
embryos in lateral view, rostral to the top and dorsal to the right. ctl,
control embryos. (A-D) lhx5 is expressed in rostral regions
during embryonic development. (A,B) Dorsal to the right, lateral (A) and
animal pole (B) views. (D) A gap can be seen between the lhx5 and
pax2a expression domains. (E-N) lhx5 gain of function
causes expansion of the forebrain. (E,F) Forebrain boundaries are marked by
broken lines. (G,H) Dorsal view, bud stage, pax6a expression. (I-N)
Dorsal view, rostral to the top. Embryos were dissected and flat mounted in
glycerol after in situ hybridization. Partial overlays of panels were made
with PhotoShop (Adobe) and brightness in the overlapped regions was adjusted
so that the backgrounds match. (O-V) Inhibition of Lhx5 function
compromises forebrain development. (U,V) Red arrowheads mark the
forebrain-midbrain boundary. (W-Z) lhx5 morpholino knockdown
alters pax6a and pax2a expression. Embryos were dissected
and flat mounted in glycerol after in situ hybridization. Dorsal view, rostral
to the top. Scale bar in Z: 250 µm for A-H,Q-V; 200 µm for I-K; 167
µm for L-N; 150 µm for O,P; 100 µm for W-Z.
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