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Fig. 2. FT transcript levels are drastically increased in SD-grown,
early flowering spa mutants. (A) RT-PCR analysis of
CO, FT, SOC1, FLC and ubiquitin (UBQ10) mRNA
abundance in wild-type (WT) and spa mutant plants. (B-E)
Quantification of mRNA abundance of the blots shown in A. The transcript
levels of flowering time genes were normalized by the transcript levels of
UBQ10. A representative experiment of two or three independent
experiments is shown. Notice the difference in scale of FT/UBQ10 in
the two graphs in C. Wild-type (Col), spa1-7, spa1-7 spa3 spa4 and
spa2 spa3 spa4 (Col) plants were grown in SD for 25 days. RNA was
isolated from these plants and used for RT-PCR analysis.
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