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Fig. 3. Phenotypic analyses of cuc and syd-2 or
atbrm-1 double mutants. (A-C,E) Pictures taken at
the indicated days after germination. None of the double mutants exhibit loss
of shoot apical meristem function with the exception of cuc2-1
atbrm-1 (not shown); shoots form from the fused region of the cotyledons
(yellow arrowheads in C-E). Genotypes are indicated in each panel. (D)
Picture of a cleared seedling taken using DIC optics. Scale bars: 2 mm in
A-C,E; 100 µm in D. (F) Percentage of plants showing cotyledon
fusion in double mutant combination with cuc1-1, cuc2-1 or
cuc3-101. Cup indicates cup-shaped seedling with substantial fusion
on both sides of each cotyledon blade (as in B and E). CPF includes seedlings
with complete fusion of one side of the cotyledon blade (see C), fusion of the
cotyledon petiole, as well as cup-shaped seedlings. atbrm-1 mutants
enhance all three cuc mutants, whereas syd-2 mutants only
significantly enhance cuc1-1. The number of seedlings tested for each
genotype is shown in parentheses.
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