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Figure 2


Fig. 2. Generation of an Osx1-GFP::Cre transgenic mouse line. (A) Schematic outlining the linear configuration of the pTGCK cassette used for homologous recombination to the first exon of the osterix1 locus contained within the BAC: RP23-399N14. The correctly targeted BAC was subsequently used to generate a transgenic mouse line by pro-nuclear injection. (B-K) Founder Osx1-GFP::Cre male transgenic mice were crossed to the female Rosa26lacZ reporter line and activity of the transgenic line was observed by way of whole-mount lacZ assay at (B) E14.5, and by lacZ assay or direct fluorescence microscopy on 15 µm cryosections of tibia from (C,D) E14.5, (F,G) E18.5 and (I,J) postnatal day 10 mice. (E,H,K) Negative control specimens for littermates, at each corresponding age, that do not carry the transgene.





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