spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Osx1-GFP::Cre removal of ß-catenin function results in a failure to develop terminally differentiated osteoblasts. (A-Q') Histological analysis and in situ hybridization of 35S-labeled riboprobes to sections of E18.5 tibia from (A-Q) ß-cateninc/n (wild type) and (A'-Q') Osx1-GFP::Cre;ß-cateninc/n (mutant) embryos. Specific anti-sense riboprobes were used that identify marker genes for: states of osteoblast and chondrocyte differentiation, (D,D') Col1{alpha}1, (E,E') Runx2, (F,F') osterix1, (G,G') osteocalcin, (H,H') Col2{alpha}1 and (I,I') Col10{alpha}1; components of Hh signaling, (J,J') Ihh and (K,K') Ptch1; components of canonical Wnt signaling, (L,L') Dkk1 and (M,M') Tcf1; the early osteoblast marker, (N,N') Bmp3; matrix remodeling, (O,O') Mmp9 and (P,P') Mmp13; and vascularization, (Q,Q') Vegf. Boxed areas in B and B' are presented at higher magnification in C and C', respectively. Black arrows indicate the absence of ossification in the mutant periosteum. White arrowheads indicate the invading wedge of mesenchymal cells into the marrow cavity, whereas black arrowheads indicate sites of ectopic expression of chondrocyte marker genes.





Right arrow Return to article