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Fig. 2. Proliferation, interkinetic nuclear migration and orientation of cell
division in wild-type and laminin 
1III4-/- cortex at
midneurogenesis. Fluorescent micrographs of E14 wild-type and laminin
1III4-/- cortex immunostained as indicated
(A,B,E-H) reveal similar numbers of precursors (Ki67-positive)
in mitosis (PH3-positive) at the ventricular surface (VS, arrowheads in A) or
at abventricular positions. Ectopic clusters of precursor cells within the
laminin 1III4-/- cortical plate (CP) are indicated by arrows
in B. (C) Histogram depicting the percentages of precursors dividing at
the ventricular surface (VZ in C), subventricular zone (SVZ) or at ectopic
positions assessed by the quantification of PH3-positive cells at E14 (wild
type: n=40 sections, two animals, laminin 1III4-/-:
n=45, two animals). (D) Histogram depicting the number of
PH3-positive cells per cortex area (100 µm2) dividing at VZ or
SVZ positions respectively (wild type: n=43, two animals; laminin
1III4-/-: n=51, two animals). (E-H)
BrdU-immunostaining (red) reveals cells in S phase (0.5 hours after
BrdU-injection; E,F) and 6 hours after S-phase labelling when they have moved
towards the ventricular surface (G,H). (I) A dividing cell in anaphase
labelled with propidium iodide. The angle of cell division was assessed by
measuring the angle between a line at the VS and the separating chromatids.
(J) Histogram depicting the percentages of cells dividing horizontally
with respect to the VS (0-30°), obliquely (30-60°) and perpendicularly
(60-90°) in E14 cortex (wild type: n=103 mitoses, two animals;
laminin 1III4-/-: n=110, two animals). Scale bars:
100 µm
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