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Figure 2


Fig. 2. ed clone borders induce the formation of a contractile actin cable and reduction in adherens junction components. (A) edF72 follicle cell clone (lack of NM marker, basal confocal section). (A') Staining with fluorescently-labeled phalloidin (apical confocal section) reveals enriched F-actin at the clone border and reduced apical circumference.(B,B') Cross-section of an edF72 mutant clone (B) stained with fluorescently-labeled phalloidin (B') illustrates the apical constriction of the clone border (arrows, apical is towards the bottom). (C) edF72 follicle cell clone (basal confocal section). (C') Increased p-MLC immunoreactivity (apical confocal section) is detected at the clone border.(D,D') Enlargement of an edF72 clone border. Two parallel lines of p-MLC immunoreactivity can be resolved. (E,E') Stage 12. Some edF72 mutantclones exhibit severely reduced or discontinuous DE-cad immunoreactivity at the clone border (arrow). (F,F') Stage 12. At edF72 mutant clone borders with a milder DE-Cad defect, DE-Cad is occasionally absent (arrow) but often discontinuous or unaffected (arrowhead).





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