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Fig. 3. Ed exhibits a spatially and temporally dynamic distribution during oogenesis. (A) Homozygous ed^F72 follicle cell clone (lack of NM marker, basal confocal section). (A') Ed immunoreactivity is absent from the ed mutant cells and absent (arrow) or discontinuous (arrowhead) from wild-type cells at the clone border (apical confocal section). (B) Ed is enriched at the apical (germline-facing) side of the tissue (inset; apical is indicated with an arrowhead, basal with an arrow) at early stages (left) then becomes undetectable by early stage 10B (right). (C) Late stage 10B (dorsal view). Ed is detected at the dorsal midline of the main body follicle cells (only the posterior half of the egg chamber is shown). (C') Same egg chamber as in C, labeled with fluorescently-labeled phalloidin. A smooth interface coincides with the endogenous Ed expression border (arrow). (D) Stage 11 (dorsolateral view). Ed is absent from two dorsolateral groups of follicle cells. (D') Same egg chamber as in D (dorsal anterior portion), labeled with fluorescently-labeled phalloidin. A smooth interface (arrow, arrowhead) corresponds to the endogenous Ed expression border.

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