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Fig. 2. FGF signaling is required for ß-catenin-induced organizer
formation. (A,B) Distribution of phenotypes of
ichabod embryos injected with RNAs for ß-catenin alone (red) or
co-injected with RNAs for ß-catenin and the inhibitor of FGF signaling
(yellow) XFD (A), MKP3 (B) or the inactive FGF receptor, d50 (light blue, A).
Uninjected ichabod embryos were severely ventralized (dark blue).
ß-catenin can partially or completely rescue most embryos, but both the
dominant negative FGF receptor, XFD (A) or MKP3(B) inhibit this rescue. The
non-functional FGF receptor, d50, had no effect on ß-catenin rescue (A).
(C-G) Typical representative embryos are shown for each condition of
treatment. (H-M) FGF signaling is essential for formation of the
organizer, as assayed by gsc expression. At 50% epiboly, wild-type
embryos robustly express this gene in the shield region (H), but
ichabod embryos are devoid of expression (I). ß-catenin
induction of gsc in ichabod embryos (J) is abolished by
coexpression of XFD (K) or by injection of SU5402 (M) but not d50(L). Embryos
in H-M are shown in animal pole views.
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