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Fig. 4. Complementary distribution of DE- and DN-cadherins during ommatidial rotation. All panels show confocal microscopy images of 3rd instar eye discs (anterior is leftwards, dorsal is upwards). (A) DE-cad (green) and Arm (red). Enriched staining is found in morphogenetic furrow (MF, white arrowhead) and within precluster cells (see also D and E for high-magnification images). Arm and DE-cad overlap, except at R3/R4 border, where DE-cad is almost absent (examples indicated by arrows). (B) Arm (red), DN-cad1 (blue). (C) Enriched DN-cad staining is present at the R3/R4 cell border (arrows in B,C; see also E for high magnification.) (D,E) High magnification of preclusters stained for DE-cad (green) and Arm (red) in D-D'', and DE-cadGFP (green), DN-cad (blue) and Fmi (red) in E-E''. DE-cad and DN-cad1 distribution is largely non-overlapping (E''). (F-I) DE-cad and Arm localization analyses in mutant backgrounds. (F) Arm (red) localization in N-cad¹⁴ clones (marked by absence of LacZ). Arm is reduced only at the R3/R4 border membrane (examples marked by arrowheads; compare with wild type marked by arrow). (G) Arm (red) and DN-cad (blue and G') in shg^P34-1 mutant tissue. Arm is almost completely lost from apicolateral membranes, except where it co-localizes with DN-cad (which is not affected). (H). Example of DN-cad1 distribution in shg^P34-1 clone (wild type marked by GFP: green): accumulation at R3/R4 border is unchanged (see arrowheads). (I-I'') Overexpression of DN-cad1 (marked by GFP; green) reduces DE-cad (blue and I'), but not Fmi (red and I'') levels.

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