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Fig. 5. DN-cad1 and DN-cad2 are required for normal ommatidial rotation. (A,B) N-cad¹⁴ double mutant clones in adult eyes (A) and eye discs (B-B''). (A) A mosaic eye containing N-cad¹⁴ clones (owing to the nature of the allele, the clone cannot be marked). Several clusters with rotation defects are present (dots in schematic indicate ommatidia with photoreceptor loss that cannot be scored for rotation). (B-B") Ventral part of eye disc with N-cad¹⁴ clone marked by absence of ßGal (green), stained for Elav (blue) and Bar (red); in semi-schematic (B''), the rotation angles are indicated with white (wild-type clusters) or yellow (mutant or mosaic clusters) bars. Most mutant clusters are over-rotated relative to their stage. (C) sev>DN-cad1 eye disc, Bar staining highlights random rotation throughout the disc (D, Bar-staining in wild-type disc for comparison). (E) Clone of cells overexpressing DN-cad1 (GFP). Localization of Arm (red and E') to plasma membranes and cytoplasmic membrane compartments is increased in the clone. (F-H) sev>DN-cad1 is suppressed by gene dose reduction in arm (sev>DN-cad1/arm⁴; compare G with F). Gaps between ommatidia resulting from decreased adhesion (although rotation defects are not enhanced by Arm decrease) and enhanced by Arm co-overexpression (sev>DN-cad1;>Arm^S2; H).

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