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Figure 1


Fig. 1. Ice{Delta}1 is a null allele of Ice. (A) Genomic organization of the Ice region. The P transposon element EPGE28489 is inserted at base 1711 in the Ice transcription unit. The Ice{Delta}1 excision line removes the entire Ice-coding region, fusing small remaining fragments of the Ice 5'- and 3'-UTR sequences. A 1.5 kb fragment of EPGE28489 also remains. The gray region indicates the Ice-coding region; the black boxes indicate the UTR sequences of Ice and of two neighboring genes. Locations of the EPGE28489 element, the Ice{Delta}1 deletion and the Ice genomic rescue construct (Ice+2.4) are indicated to scale. (B) Western blot of adults of various genotypes probed with anti-Ice antibodies. The genotypes are wild type, Ice{Delta}1, Ice{Delta}1; Ice+2.4 and dcp-1Prev1 ({Delta}Dcp-1), a null allele of dcp-1.





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