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Figure 6


Fig. 6. Ice is required for cell death induced by X-irradiation and inhibition of protein synthesis. (A-F) TUNEL-stained third instar wing discs of various genotypes. (A) Untreated wild type; (B) X-irradiated wild type; (C) untreated Ice{Delta}1; (D) X-irradiated Ice{Delta}1; (E) X-irradiated Ice+2.4; Ice{Delta}1; (F) X-irradiated dcp-1Prev1. (G) Wild-type hemocytes exposed to cyclohexamide died within 4 hours. Removal of zygotic Ice (Ice{Delta}1)prevented almost all this death, which was restored in the presence of a wild-type Ice rescue transgene (Ice{Delta}1,Ice+2.4). Animals homozygous for Ice{Delta}1 lack zygotic but not maternal Ice. Animals homozygous for dcp-1Prev1 lack both maternal and zygotic dcp-1.





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