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Figure 4


Fig. 4. Hematoxylin and eosin staining reveals complete palate fusion and seam degeneration in Apaf1 mutant embryos. (A,D) The MEE seam (arrowhead) forms normally in both wild-type and Apaf1 mutant embryos at E14.5. (B,E) The MEE seam undergoes degeneration in both wild-type and Apaf1 mutant embryos at E15.5 to establish the mesenchyme confluence cross the midline (arrowheads). (C,F) At E16.5, both wild-type and Apaf1 mutant embryos form a continuous palate with no sign of seam cells in the midline area (arrowhead).





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