Actin capping protein maintains vestigial-expressing cells within the Drosophila wing disc epithelium

DEV02511 Supplementary Material

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• Supplemental Figure 1 -

  Fig. S1. Dynein/Dynactin-based transport, JNK and blistered are not essential for extrusion of cpa mutant cells. Optical cross sections through the wing disc epithelium of clones positively labeled with GFP (green) and stained with anti-Dlg (blue in A,B,C,F,G,H or red in D,E) and anti-Caspase 3 (red in A,B,C,G,H) or anti-Arm (red in D,E). (A) cpa^69E mutant clones. (B) khc^K13314 mutant clones. (C) cpa^69E; khc^K13314 double mutant clones. (D) Clones overexpressing bsk^DN. (E) cpa^69E mutant clones overexpressing bsk^DN. Removal of bsk or khc fails to rescue extrusion of cpa mutant clones. (F) Clones expressing Mal-D-ΔN. Removal of the N terminus of Mal-D renders it nuclear and active (Somogyi and Rorth, 2004). (G) Clones expressing dia^CA. (H) cpa^69E, bs² double mutant clones. Expression of Mal-D or dia^CA causes extrusion and death of epithelial cells, but removing dSRF fails to rescue extrusion of cpa mutant cells. The white arrows indicate the wing blade region.