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Fig. 6. The N terminus of Slug binds to Ppa and can transfer this interaction to
Sox10. (A) Embryos were co-injected with Ppa and wild-type Slug,
Slug-N or Slug-C. Following immunoprecipitations using an 
-Myc antibody
(Slug/mutants), bound Ppa (arrow) was detected by -Flag immunoblot. Ppa
binds the N terminus of Slug, but not the C terminus. (B) Embryos
co-injected with Ppa and Slug, Sox10 or a Slug N-Sox10 fusion construct were
collected for IP/western blotting. The addition of Slug M1-H64 is sufficient
to transfer Ppa binding to Sox10 (arrow). (C) Serial Slug deletion
mutants were co-injected with Ppa to map the Ppa binding region. (D)
Co-immunoprecipitation experiments showing that a Slug mutant with deletion of
amino acids P31 to H64 no longer interacts with Ppa.
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