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Fig. 4. Khc mutants resemble milt class II mutants. (A-C) Prefollicular cysts: mutant Khc²⁷ clonal cysts (B, broken outlines) show premature mitochondrial accumulation (green, arrow) at the middle of the fusome (magenta) in contrast to wild-type (A) and Dhc64C^6-6/6-12 (C) cysts. (D-F) Forming follicles: the Balbiani body (broken yellow line) is greatly enlarged in Khc²⁷ clones (E) and reduced in Dhc64C^6-6/6-12 mutants (F) compared with wild type (D). The Dhc64C^6-6/6-12 oocyte (F inset, barbed arrow) can be distinguished from the nurse cells by its selective accumulation of Cup protein (blue). (G-J) Dynein heavy chain (green) localization is indistinguishable between wild type (G,H) and milt^EY01559 (I,J), both during follicle formation (G,I) and in older follicles (H,J). By contrast, Dhc accumulates in intense puncta in the posterior region of Khc²⁷ mutant oocytes (arrows, K,L). A wild-type oocyte shows normal diffuse Dhc localization (L, barbed arrow). (A-F) ATP synthase, green; (G-L) Dynein heavy chain, green; (A-J) 1B1, magenta; (B,E,K,L) ß-galactosidase marks wild-type cells (blue). Scale bars: 10 µm in A-F; 10 µm in G,I,K; 20 µm in H,J,L.

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