QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Mitochondrial mislocalization in milt class II mutants reveals microtubule polarity. (A) Known apical polarity of microtubules in follicular epithelial cells. (B,C) Mitochondria (green) are evenly distributed in wild type (B, arrow) and milt⁹² mutant (C, broken outline) follicle cells. (D,E) Mitochondria cluster at the apical side of milt^k06704 mutant follicle cells (D, arrow), as predicted (A). Khc²⁷ mutant follicle cells also accumulate mitochondria at their apical surface (E, arrow) until stage 7, when mitochondrial distribution becomes normal (E, asterisk). (F,G) Mitochondria predominantly localize at the anterior end of wild-type (F, arrows) and milt⁹² mutant (G, arrow) GSCs (broken outlines) near the spectrosome (magenta). By contrast, in milt^k06704 mutant clones (H, arrow) and Khc²⁷ mutant clones (I, arrow) mitochondria aggregate at the posterior of the GSC (broken outlines). This suggests the minus ends of the microtubules are directed towards the posterior of the stem cell, away from the spectrosome (J). (K-M) Mitochondria in dividing cysts are normally spread evenly throughout the cytoplasm (L, broken outline). However, they clump away from the fusome (magenta) in Khc^{2 7} clones (I, barbed arrow; M, arrows), indicating the location of microtubule minus ends (K). (B-I,L,M) ATP synthase, green; (B,F,G,I,L,M) 1B1, magenta; (C-E,H,I,M) ß-galactosidase marks wild-type cells (blue). Scale bars: 10 µm in B-E; 10 µm in F-M.

Return to article