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Fig. 1. Identification, predicted primary sequence, homology and expression of
Zfp488 mRNA transcript in the brain. (A) Differential gene
expression between wild-type and Olig1-null (
KO)
optic nerves was examined by mRNA differential display with duplicate samples.
Arrows indicate the differentially expressed genes between wild-type and
Olig1-null optic nerves. Red arrow indicates the position for
Zfp488. (B) Zfp488 expression in the brain of
wild-type and Olig1-null mice at P14. Upper panel, a northern blot of
RNA extracted from brain tissues of wild-type and Olig1 mutant mice
was probed with 32P-labeled Zfp488, revealing a 
3.5
kb mRNA transcript in the wild-type brain and its absence in the
Olig1-null brain. Lower panel shows expression of the housekeeping
gene Gapdh examined by semi-quantitative RT-PCR (20 cycles) as a
loading control. (C) Predicted amino acid sequence of mouse
Zfp488 protein, showing C2H2 type (boxed) zinc-finger domains in the
C-terminal region. Potential nuclear localization signals are underlined.
(D) Alignment of evolutionarily conserved zinc finger domains of
Zfp488 among invertebrates and vertebrates.
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