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Figure 4


Fig. 4. Zfp488 expression is absent throughout the CNS in the Olig1-null mice. (A-F) Expression of Zfp488 mRNA was analyzed in situ on the spinal cord, the corpus callosum and the cerebellum taken from wild-type (A,C,E) or Olig1{Delta}KO (B,D,E) mice at E18.5 and P14 as indicated. (B,D,F) Absence of Zfp488 expression in the spinal cord (SC), the corpus callosum (CC) and cerebellum (CBM) was observed, indicating that Zfp488 expression requires Olig1. Arrows indicates Zfp488 expression in the white matter of SC (A), CC (C) and CBM (E), respectively. (G) Luciferase reporter activity driven by a 3.2 kb Zfp488 upstream regulatory region in the pGL3 vector indicates that transfection of Olig1 activates Zfp488 expression by approximately fourfold (P<0.01). By contrast, Olig2 activity on the putative promoter is statistically insignificant (P>0.05) using the Student's t-test. Data are derived from at least three independent experiments and presented as the mean±standard deviation.





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