DEV02497 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Bmp4 heterozygous mutant embryos have reduced allantois length. (A) Allantois length (μm) was measured in embryos from F1(C57BL/6J×CBA) females mated with a heterozygous Bmp4 male on the C57BL/6J×CBA background. Results were expressed as means±s.e.m. in each group. A numerator indicates the embryos with an allantoic bud. Zero values are not included in the mean. The initiation of the allantois is delayed (LSEB) in the heterozygote (P<0.01 on Wilcoxon’s Summed Ranks test), but the rate of extension parallels that of the wild-type thereafter. (B) The ratio of the allantois length measured in A over the embryonic axis length.

• Supplemental Figure 2 -

  Fig. S2. Gene dosage effect of Smad5 on PGC numbers. Regression analysis of PGC number (counted in whole mount) versus somite number in embryos from Smad5^m1/+ intercrosses, not including PGC-like cells in the amnion. Values on C57BL/6J and F2 (C57BL/6J×CBA) backgrounds did not differ and have been pooled. The values in the linear regression equation, Y=a+bX, for log PGC (Y) on somite (X) number at the mean values of X and Y with each set of data: wild type, log 112.6=log 49.41+0.02817 (12.70); heterozygote, log 78.83=log 34.91+0.02710 (13.05); homozygous mutant, log 22.88=log 7.956+0.03359 (13.65). The slopes of the three regression lines are not significantly different, indicating an absence of effect of Smad5 on PGC proliferation. The difference in elevation of the regression lines of heterozygote compared with wild type (P<0.001) and homozygous mutants with heterozygotes (P<0.001) indicates a gene dosage effect on the size of the founder population.