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Figure 5


Fig. 5. Ectopic Bmp signalling in Smad5m1/m1 amnion. (A-C) Anti-P-ERK1/2 antibody staining can barely be detected in the amnion (arrowhead) at E8.5 (A). It is increased in non-thickened Smad5 mutant amnion but especially elevated in the aggregate of cells (B,C). B and C represent two different Smad5 mutants. (D-F) Anti-phospho-Smad1/5/8 antibody staining can only be detected sporadically in an isolated cell of the amnion (arrowhead) at E8.5 (A). P-Smad1/5/8 is upregulated in non-thickened mutant amnion and within the aggregates of Smad5 mutant amnion. Sections in D-F are sister sections of A-C. (G,H) The Bmp target gene periostin is expressed in the amnion (arrowhead) at E8.5. It is strongly upregulated in the non-thickened Smad5 mutant amnion, although excluded from the aggregate of cells (H). (I,J) The Bmp target gene Msx2 is barely detectable in wild-type amnion (I) but highly expressed in mutant amnion at E8.5 (J). Msx2 expression is primarily confined to the ectodermal component of the amnion (arrowhead) and the aggregate of cells (arrow). The expression of Msx2 in the embryo proper is not altered significantly. AmMe, amnion mesoderm; He, heart; Nt, neural tube; Se, surface ectoderm; Ys, yolk sac. Scale bars: 100 µm.





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