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Fig. 6. Local administration of rBMP4 induces thickening of the amnion in
wild-type embryos. (A,B). Lateral view of wild-type embryos
that were either control injected (A) or injected with rBMP4 (B) in the
exocoelom at the EHF stage (E7.5), and cultured in vitro until the 5S stage.
The amnion (arrowhead) developed a local thickening in the rBMP4-injected
embryo (dashed circle). Aggregates were always much smaller than in
Smad5 mutants, and difficult to see through the yolk sac. (C-F)
Sections through control (C) and rBMP4-injected (D-H) embryos.
The appearance and size of the amnion thickening (dashed circle) varied
significantly between embryos. Thickening of the amnion was only scored when
observed in more than five successive sections. The embryos in C-E were
injected at the HF stage; D and E are sections from the same embryo,
illustrating that amnion thickening can develop at different locations. The
embryos in F-H were injected at the NP stage. The embryo in H has an unusually
large yolk sac. Al, allantois; Fg, foregut; He, heart; Nt, neural tissue; Ys,
yolk sac. Scale bars: 100 µm.
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