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Figure 5


Fig. 5. The postmigratory germ cell marker GCNA1 is ectopically expressed in somatic cells of Dnmt1-deficient embryos. All embryos were immunostained for GCNA1 expression. (A) 9.5 dpc Dnmt1+/n embryo. (B) Enlarged view of the same embryo. (C) 9.5 dpc Dnmt1n/n embryo (n=4/4). (D) Enlarged view of embryo in C, demonstrating scattered GCNA1 expression throughout the embryo. (E) 9.5 dpc Dnmt1+/c embryo. (F) 9.5 dpc Dnmt1c/c embryo (n=5/5). (G) Enlarged view of embryo in F. (H) 8.5 dpc Dnmt+/c embryo. Micrographs in A,C,E,F are at the same magnification. Ectopic GCNA1 expression was not detected in any of 11 control littermate embryos stained in parallel (not shown).





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