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Fig. 3. Blocking FGF signal transduction with SU5402 (10 µM final) in
wild-type limb buds (E10.5, 34-36 somites) phenocopies aspects of the
disruption of Jag1 and Hoxd13 expression in
Grem1-deficient limb buds. (A-D) Asterisks indicate the
anterior margins when necessary. (A) Jag1 expression in a wild-type
forelimb bud cultured for 16 hours. Left panel: dorsal view with posterior
towards the bottom and distal towards the left. Right panel: view onto the
distal part of the same limb bud to reveal the anterior expansion of
Jag1 expression at this stage. (B) Jag1 distribution in a
wild-type forelimb bud cultured with SU5402 for 16 hours. Left panel: top view
onto the distal limb to allow direct comparison with the control. Right panel:
dorsal view. (C) Hoxd13 expression in a wild-type forelimb bud
cultured for 16 hours. Left panel: dorsal view. Right panel: top view. (D)
Hoxd13 distribution in a wild-type forelimb bud cultured with SU5402
for 16 hours. Left panel: top view of the distal limb to reveal the
downregulation of Hoxd13 expression and lack of anterior expansion in
the distal mesenchyme. Right panel: dorsal view. (E,F)
Jag1 (E) and Hoxd13 expression (F) in wild-type and
Grem1 deficient (Grem1In2/In2) forelimb buds at
E11.0 (42 somites, not cultured), which corresponds to a stage similar to the
limb buds shown in A-D.
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