Supplemental Figure 1
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Fig. S1. Localization of phospho-MAPK1/MAPK3 and phospho-MAP2K1/MAP2K2 in wild-type and Map2k1−/− placentas. Anti-phospho-MAPK1/MAPK3 and anti-phospho-MAP2K1/ MAP2K2 staining of E9.5 (A) and E10.5 (B) wild-type, Map2k1−/− and Map2k2−/− placentas. (A) At E9.5, MAP2K1/MAP2K2 were phosphorylated in cells lining the maternal sinuses (black arrows), in cells of the allantois (red arrowheads) and in some labyrinthine trophoblasts of wild-type, Map2k1−/− and Map2k2−/− placentas. However, phospho-MAPK1/MAPK3 signal was primarily found around maternal sinuses (black arrows) and in some labyrinthine trophoblasts in wild-type and Map2k2−/− specimens. In Map2k1−/− placentas, MAPK1/MAPK3 were activated only in the allantois (red arrowhead). (B) At E10.5, phospho-MAP2K1/MAP2K2 signal was also observed in the vascular endothelial cells lining the embryonic blood vessels (black arrowheads). Despite the high activation of MAP2K2 at the chorioallantoic interface (red arrows) and in the allantois of Map2k1−/− placentas (red arrowhead), phospho-MAPK1/MAPK3 were detected only in the allantois (red arrowhead). al, allantois; ev, embryonic blood vessel; g, tropohoblast giant cells; l, labyrinth; ma, maternal sinus. Scale bar: 50 μm.
