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Fig. 4. Abnormal vascularization of Map2k1-/- placental
labyrinth. The embryonic vascular network of E10.5 wild-type (A)
and Map2k1-/- (B) placentas was revealed by
anti-CD31 staining (black arrows). In Map2k1-/- placentas,
the fetal blood vessels were restricted at the chorioallantoic plate (B).
Detection of p38 MAPK activation in wild-type (C) and
Map2k1-/- (D) placentas revealed a high activation
of p38 MAPK in the embryonic blood vessels of the labyrinth of wild-type
specimens and at the chorioallantoic interface of
Map2k1-/- placentas (black arrowheads). Vegf
expression in wild-type (E) and Map2k1-/-
(F) placentas was analyzed by in situ hybridization (red signal and
blue arrows). High Vegf expression levels were detected in the
allantoic region of Map2k1-/- placentas, indicating a
response to hypoxic stress. For placental structure identification, near
adjacent Hematoxylin and Eosin-stained sections of specimens E and F are
presented in Fig. 1A. X-Gal
staining of Map2k1+/- (G) and
Map2k1-/- (H) placentas was used to define the
Map2k1 expression profile in normal and mutant placentas. The
labyrinthine region is presented. In Mapk2k1-/- specimens,
X-Gal staining is restricted to the chorioallantoic plate. al, allantois; ch,
chorionic plate; d, deciduum; g, tropohoblast giant cells; l, labyrinth. Scale
bars: 50 µm in A-D,G,H; 100 µm in E,F.
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