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Fig. 2. Accumulation of functional UNC-5::GFP in the cell bodies of DD/VD neurons in unc-51 and unc-14 mutants. (A-E) Localization of UNC-5::GFP (A,B,D) and of mRFP (C,E), which marks cell bodies and axons of DD/VD neurons. (A) Wild type. (B,C) The same region in unc-51(e369). (D,E) The same region in unc-14(e57). Arrowheads point to DD/VD cell bodies. Open triangles point to the abnormal accumulation of UNC-5::GFP. Scale bar: 10 µm. UNC-5::GFP was localized to small vesicles throughout the axons and cell bodies in wild-type animals (A). UNC-5::GFP accumulated in the cell bodies of DD/VD neurons in unc-51 (B) and unc-14 (D) mutants. A small amount of UNC-5::GFP was present in axons (B). (F,G) Quantification of GFP fluorescence at cell bodies (F) and axons (G). In each case, 20 cell bodies and axons were examined and the results were averaged. The numbers at the bottom are in arbitrary units that were measured by using an LSM510 confocal microscope (Zeiss). Error bars show the standard error. ^* P<0.01 (Bonferroni correction). NS, not significant. We determined their cell bodies and axons by using the fluorescence of mRFP. In unc-51(e369) mutants, large amounts of UNC-5::GFP accumulated in cell bodies. In addition, the amount of UNC-5::GFP in axons reduced. In unc-14(e57) mutants, although certain accumulation of UNC-5::GFP was observed in the cell bodies (D), the fluorescence amounts of the cell bodies and the axons were statistically not different from that of wild type.

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