DEV02496 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Fig. S1. PUF-8::GFP and FBF-2::GFP expression analysis. (A) Semi-quantitative time-course analysis of PUF-8::GFP expression in wild-type animals. The two daughter cells after the first cell division are termed Pn.px for all VPCs. The descendants of the second cell divisions of induced VPCs are termed Pn.pxx, and after the third round of cell divisions, Pn.pxxx cells. (B,C) Analysis of PUF-8::GFP expression pattern in (B) eff-1(hy21) mutants at the Pn.px stage with unablated (row above) and with ablated gonads (row below), and in (C) let-60(n1046gf) animals at all stages. (D) Semi-quantitative time-course analysis of FBF-2::GFP expression in wild-type animals. Only animals showing bright FBF-2::GFP expression in somatic tissues were used for the analysis. In all graphs, white indicates no expression, grey low expression and black high expression.

• Supplemental Figure 2 -

  Fig. S2. Germ line and somatic gonad-independent vulval induction in fbf-1 fbf-2; gap-1 animals. (A-D) One example for each class of ablations referred to in Table 3 is shown. (A) fbf-1(ok91) fbf-2(q704); gap-1(ga133) L4 stage animals with intact gonads (mock), (B) with ablated germ cells (Z2 and Z3), (C) with ablated somatic gonad (Z1 and Z4) and (D) with complete gonad ablation (Z1 though Z4). Arrowheads show the normal vulva consisting of the three proximal VPC descendants (P5.p, P6,p and P7.p), arrows indicate ectopic vulval induction formed by distal VPCs. In all panels, anterior is to the left and ventral is to the bottom. Scale bar in D: 10 μm.