QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Deletion of IKK2 in mammary gland results in reduced apoptosis and delayed involution. (A) Haematoxylin and eosin staining of mouse mammary gland sections from Cre^-, Cre⁺/Ikk2^fl/wt or Cre⁺/Ikk2^fl/fl mice at 0dL, 24, 48, 72 or 144 hours' involution. Apoptosis is apparent in glands from Cre^- control mice that subsequently underwent remodelling normally. Apoptotic cells are seen to accumulate in the open lumen of the lobuloalveolar structures (<). Involution and remodelling was delayed in the glands from Cre⁺/Ikk2^fl/wt mice. This delay was more pronounced in Cre⁺/Ikk2^fl/fl glands. By 6 days' involution very little difference is seen between mice of the different genotypes. (B) Cleavage of caspase 3 was determined by immunofluorescence in mammary gland sections from Cre^-, Cre⁺/Ikk2^fl/wt or Cre⁺/Ikk2^fl/fl mice at 24, 48 or 72 hours' involution. (green: cleaved caspase-3-positive cells; red: DAPI-stained nuclei). TUNEL staining at 72 hours' involution showed a higher proportion of positive (green) cells in Cre^- and heterozygous glands. (C) Cleaved caspase-3-positive cells were expressed as a percentage of total cell number and data presented in graphical format. Approximately 10,000 cells were counted. Cleaved caspase 3 levels peaked at 24 hours' involution in Cre^- glands, was delayed by 48 hours in Cre⁺/Ikk2^fl/wt mice and remained constant at all involution timepoints in Cre⁺/Ikk2^fl/fl mice. Scale bars: 100 µm.

Return to article