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Figure 1


Fig. 1. CDC-42 is required for the PAR-2 localization cycle. (A-C) Time-lapse images of GFP-PAR-2 polarity establishment in (A) control, (B) cdc-42(RNAi) and (C) rho-1(RNAi) embryos. Times (seconds) are relative to nuclear envelope breakdown. In this and subsequent figures, the embryos are approximately 50 µm in length; the embryo posterior is to the right. (A) In control embryos, GFP-PAR-2 localizes uniformly along the cortex around the time of meiosis (top). After meiosis, GFP-PAR-2 disappears from the cortex (middle) and becomes confined to the posterior pole (bottom). (B) In cdc-42(RNAi) embryos, GFP-PAR-2 localized uniformly at the cortex. (C) Rho-1(RNAi) did not affect the PAR-2 localization cycle.





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