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Fig. 8. Loss of SG1 and SG2 affects the quality and quantity
of lumenal secretory content. (A-C) TEM analysis of wild-type
(Oregon R) and Df(3R)Exel6216 salivary glands revealed differences in
both the volume and morphology of apical secretions. (A-A''') Thin
sections of wild-type salivary gland cells revealed uniform apical cell
surfaces surrounding a relatively large lumenal space filled with fibrillar
matrix material (asterisks). (B-B''') Thin sections of
Df(3R)Exel6216 salivary glands revealed irregular and small lumenal
spaces containing matrix of increased density (asterisks) and large regions
where the apical cell surfaces appear to meet. (C,C') New adherens
junctions (AJs) appear to have formed at the contact site of cells arising
from opposite sides of the lumen of Df(3R)Exel6216 salivary glands
(arrowheads indicate AJs). Secretory vesicle number and morphology were
similar in wild-type and Df(3R)Exel6216 salivary glands, as were
other aspects of salivary gland cell morphology. A-A''', B-B''' and
C-C' are images from the same gland showing increased magnification from
left to right; in some cases the angle of the image is changed slightly from
panel to panel. Boxed regions in A'', B'' and C correspond to
magnified images shown in A''', B''' and C', respectively.
Df(3R)Exel6216 was balanced over TM3, twi-GFP, allowing us
to select homozygous deficiency embryos prior to fixation.
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