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Fig. 2. SPL3 promotes vegetative phase change and floral induction and
is repressed by miR156. (A) Structure of constructs used
in this study. The sequence of the mutated miR156 target site is
illustrated. A wild-type site is indicated by a black line and a mutated site
is indicated by a grey line. (B) GUS expression (top) and RT-PCR of
GUS mRNA (bottom) in progeny of crosses between transgenic plants
constitutively expressing miR156a, and miR156-sensitive or
insensitive versions of the GUS-SPL3 mRNA. (C)
Morphology of transgenic plants expressing miR156-sensitive
(SPL3) and miR156-insensitive (SPL3m, SPL3
)
versions of SPL3 under the regulation of the 35S promoter. (D)
The number of leaves without abaxial trichomes (black), with abaxial trichomes
(grey), cauline leaves (white) and flowering time (days after planting, top of
bar) for the genotypes illustrated in C (± s.e.m.). Plants transformed
with 35S::SPL3 are not significantly different from control plants.
35S::SPL3m and 35S::SPL3 have significantly fewer
juvenile, adult and cauline leaves than control plants (n>30 for
each genotype, P<0.01). (E) The effect of
35S::SPL3m on the morphology of leaves 1 and 2. This transgene
produces a significant decrease in the length of the petiole and a slightly
more acute leaf base (n=30 for each genotype;
P<0.01).
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