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Fig. 2. Melanocyte lineages develop to late stages in the presence of MoTP.
(A-H) The development of melanocyte lineages in MoTP-treated (B,D,F,H)
and untreated (A,C,E,G) larvae were examined by whole-mount RNA in situ
hybridization with mitf (A,B), kit (C,D), tyr (E,F)
and dct (G,H). For these experiments, embryos were incubated in MoTP
solution from 14 to 27 hpf, then immediately fixed for in situ analysis. The
developmental patterns and the numbers of cells (white arrowheads) for each of
the markers are indistinguishable in the MoTP-treated larvae from those in
untreated larvae. Untreated larvae were reared in PTU to completely block
melanin synthesis, thereby allowing easier visualization of NBT/BCIP
precipitates in melanocytes. Scale bar: 500 µm.
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