DEV02535 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Chromosomal positions of the spermidine synthase genes SPDS1 and SPDS2, the spermine synthase genes ACL5 and SPMS, and the four sac mutations identified in this study in Arabidopsis. The polyamine biosynthetic genes are arranged on chromosomes according to their locations in the Arabidopsis genomic sequence. sac mutations were mapped using CAPS, SSLP and SNP markers. The chromosomes are labeled with Roman numerals.

• Supplemental Figure 2 -

  Fig. S2. Suppression of the acl5 phenotype by transgenic manipulation of the SAC51 gene. (A) Recapitulation of the sac51-d mutant phenotype. Shown here (from left to right) are 6-week-old plants of wild type (WT), acl5-1, sac51-d acl5-1, acl5-1 carrying the sac51-d genomic fragment (SAC51R), acl5-1 carrying the SAC51 5′-leader deletion construct (SAC51Δ5′), and wild type carrying SAC51Δ5′. (B) Heat shock-dependent complementation of the acl5-1 phenotype. cDNA fragments of SPMS, SAC51Δ5′ and ACL5 were fused with the Arabidopsis HSP18.2 heat-shock gene promoter and introduced into acl5-1 plants. Heat-shock treatments were carried out at 37°C for 30 minutes every other day, starting 18 days after germination for a period of 2 weeks.