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Fig. 5. Map-based cloning and sequence analysis of SAC51.
(A) The region of chromosome 5 containing SAC51. The
chromosome is depicted by the uppermost horizontal line with the franking
markers LFY3 and m555. Below this are three P1 or BAC clones: MHJ24, MSJ1 and
T12B11. The markers (see Materials and methods) and number of recombinants are
shown. (B) Structure of the SAC51 gene. Boxes indicate exons
and the solid lines between boxes indicate introns. A black box represents a
principal ORF and gray boxes represent uORFs. The location of the
sac51-d mutation is shown. (C) The wild-type (Col-0) genomic
DNA sequence of SAC51 in which the regions corresponding to the cDNA
shown in uppercase letters (GenBank Accession number: AY062561). The deduced
amino acid sequences of the uORFs and main ORF are indicated below the
nucleotide sequences. Asterisks indicate stop codons and the arrowhead
indicates the position of the sac51-d mutation. The SAC51 bHLH domain
is boxed. (D) Alignment of the bHLH domain of SAC51, its homolog
At5g09460 and three characterized proteins from Arabidopsis: SPATULA
(SPT) (Heisler et al., 2001),
PHYTOCHROME INTERACTING FACTOR3 (PIF3) (Ni
et al., 1998) and TRANSPARENT TESTA8 (TT8)
(Nesi et al., 2000). Black
blocks indicate residues identical to the SAC51 sequence; gray blocks indicate
similar amino acids.
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