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Figure 1


Fig. 1. Colonization and maintenance of LTR-HSCs in the FS. (A) Sub-lethally irradiated Rag{gamma}c-/- mice (H-2k) were injected with one or two embryo equivalents (ee) of E13 FL, or with one, two, four, eight or 16 embryo equivalents of E13 FS, explanted from C57BL/6 embryos (H-2b). After 6 months, recipient BM was scored for the presence of H-2b+ donor-derived cells. Gr-1/Mac1 FACS plots are gated on live donor-derived H-2b+ cells to show the contribution of donor cells to the myeloid compartment. (B) E13 FL and FS explants (CD45.2) were cultured on filter for 4 days. FLOC and FSOC cell suspensions were injected into sub-lethally irradiated Rag{gamma}c-/- recipients (CD45.1). After 5-6 months, recipient BM and spleen were scored for the presence of donor-derived (CD45.2+) B and myeloid cells (n=6). (C) E13 FL and FS explants (CD45.2 H-2b) were irradiated, reconstituted by the same number of E10.5 AGM cells (total of six embryo equivalents of CD45.1 H-2b) and, after 4 days, FSOC and FLOC (3/injection) were dissociated and tested for LTR in Rag{gamma}c-/- (CD45.1 H-2k). The BM of recipients was scored for the presence of AGM-HSC-derived B (CD19) and myeloid (granulocytes, Gr-1) cells (n=6).





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