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Fig. 2. AGM cell differentiation potential in FL and FS environments.
(A) Differentiation of AGM-HSCs in the FS environment.
CD45.1+ FS explants were seeded with E10.5 CD45.2+ AGM
cells. After 8 days on filters, FSOC were scored for the presence of
donor-derived B (CD19) and myeloid (Mac1) cells (n=12). Plots are
gated on live PI- cells. (B) Differentiation of E13 FL
precursors in the FS environment. CD45.1+ FS explants were seeded
with E13 FL Ter119-depleted cell suspension (CD45.2+;
n=12). Analyses are identical to A. (C) Differentiation of
AGM-HSCs in the FL environment. CD45.2+ FL explants were seeded
with E10.5 CD45.1+ AGM cells (n=12). In this
microenvironment, AGM-HSCs could differentiate into CD19+ B cells,
a few of them reaching the mature IgM+ stage by the end of the
coculture (7 days only). Plots show cells gated on live donor-derived
(CD45.1+ PI-) cells.
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