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Fig. 5. Ontogeny of macrophage populations in the spleen. (A) CD45⁺ FS cells were gated and then the presence of macrophages was scored at different time-points [E15, newborn (NB), 5 days after birth (P5) and adulthood], by the expression of F4/80 and Mac1 molecules. At E15, macrophages account for half of the FS cell suspensions. (B) E15.5 FS (explanted from Rag2^GFP embryos) was analyzed by immunohistochemistry for the presence and localization of F4/80 macrophages (red, arrows). Sections were observed with a Zeiss Axioplan 2 imaging microscope and the section shown is representative of many independent FS sections. We could determine no organization between macrophages (red), lymphoid progenitors (green, arrowheads) and erythrocytes-anucleated (delineated zones) or nucleated (asterisks)-although these latter seem to be in very close contact with macrophage. Scale bar: 10 µm.

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