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Figure 3


Fig. 3. Cx43{alpha}1 modulation of cell protrusive activity. (A) Time lapse image stacks obtained from individual Cx43{alpha}1+/+, Cx43{alpha}1-/- and CMV43 CNCs captured at 5-minute intervals. Area of expansion or positive flow is indicated in red; area of contraction or negative flow is indicated in green. The image on top represents the cell at the last two frames, with underlying cell outlines corresponding to the preceding 10 timelapse imaging frames. (B,C) Cell protrusive activity in Cx43{alpha}1KO (B) and CMV43 (C) CNCs was quantitatively assessed by measuring positive (green) and negative (red) cytoplasmic flow. Roundness was also analyzed, which provided a measure of cell shape and was calculated using formula 100% x 4{pi} (area/perimeter2), with the maximal roundness being 100% for a circle. The Cx43{alpha}1KO cells showed increased positive and negative flows, together with decreased roundness (B), while no change in positive/negative flow or roundness was seen for the CMV43 CNCs (C). *P<0.05 when compared with Cx43{alpha}1+/+ or nontransgenic controls. The numbers of cells analyzed are indicated in the bars.





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