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Figure 4


Fig. 4. Rescue of zbp-89 morphants by zebrafish scl sense RNA. (A-F) Gata1-driven GFP in 22 hpf transgenic (gata1:EGFP) zebrafish embryos that were untreated (A,D), injected with atgMO only (B,E), or injected with atgMO plus scl RNA (C,F) at the one- to two-cell stage. (A-C) Single 22 hpf embryos examined under a fluorescent microscope revealing gata1 expression (red arrows) in the ICM of the untreated (A) and scl-rescued (C) embryos, but not in the atgMO only-injected (B) embryos. (D-F) Bright-field low magnification images of the respective untreated and treated Tg gata1:EGFP embryos at 48 hpf. Short arrows in E point to pericardial edema in the zbp-89 morphants.





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