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Fig. 4. Postnatal mammary expression of Gli2-lacZ in 4 day and
5-week-old virgins. Strong X-gal staining is seen in the stromal cells
(arrows) surrounding the mammary ducts in glands of 4-day-old virgin mice
(4dV) (A,B). Weaker expression is seen in the myoepithelial
cells identified by p63 immunohistochemistry (arrowheads, B). In 5-week-old
virgins (5wV), Gli2-lacZ is found in the stromal cells encasing the
ducts and is especially strong in the condensing stroma around the terminal
end bud (C,E, arrow). Expression is also found in lymphatic
ducts at all stages of mammary development (E, asterisk). Cross-section
through ducts (D) and terminal end buds (F) of 5-week-old mice
show spikes of stromal cells (arrows) between the adipocytes and myoepithelial
cells (D,F). Immunohistochemistry for p63 (G) and K14 (H)
demonstrates that, at this developmental stage, all Gli2-lacZ
staining lies beneath the myoepithelial layer (arrowheads) and is, thus,
stromal.
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