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Fig. 4. Cortically implanted AHNPs adopt predominantly neuronal fates.
(A) Coronal section of engrafted left hemisphere shows
ß-III-tubulin+/HNA+ donor cells adjacent to
engraftment site. Schematic representation includes two-dimensional
proximodistal and lateral distribution of the majority of AHNPs and
ectopically migrating cells in two transplanted animals (blue and yellow).
(B) Fate analysis indicates few cells adopt an astroglial identity.
(C) Integrating AHNPs within the primary engraftment site adopt
neuronal morphologies and immunophenotypes. (D)
ß-III-tubulin+ cells present within the hippocampus of
engrafted animals occasionally displayed HNA (E, from boxed area in d)
in CA1 and CA3, where they adopted apparent pyramidal neuron morphologies.
(F,G) Single plane confocal image of cortically implanted AHNPs.
HNA+ cells form mature neuronal (NeuN+) cell types,
which co-exist with endogenous neurons (arrowheads). Scale bars: 200 µm in
A,B,D; 50 µm in C,F,G; 100 µm in E.
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