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Fig. 3. Deregulation of Notch signaling molecules in
COUP-TFI-/- cochlea. (A-C') In situ
hybridization on sections of wild-type (A-D,I-L,Q-R,V-Y) and
COUP-TFI-/- (E-H,M-P,T,U,Z-C') cochlea for
Jag1 (A-H), Hes5 (I-P), and Lfng (Q,R,T-C'). (A-D)
At E15.5, Jag1 in wild type was predominately expressed in the region
where the non-sensory cells (arrow) are differentiating. (E-H) In
COUP-TFI-/- cochlea, there was a lack of upregulation of
Jag1 in the supporting cells region. (I-P) Hes5 expression
in wild type (I-L) was restricted to the Deiter's supporting cells (SC) of the
sensory epithelium, whereas in COUP-TFI-/- mutants (M-P),
Hes5 expression was downregulated. (Q,R,T-C') At E14.5, there
was a dramatic expansion in the Lfng expression domain (compare R and
U, circles); at E15.5, expansion to the greater epithelial ridge (GER) was
observed only in the wild type (W-Y), whereas expansion to the lesser
epithelial ridge (LER) was also observed in COUP-TFI-/-
mutants (A'-C'). (S) Immunofluorescence staining of COUP-TFI
(
-COUP-TFI) in wild-type sensory epithelium at E14.5 (arrowhead marks a
hair cell). Scale bar: in A,E,I,M,Q,T,V,Z, 200 µm; in
B-D,F-H,J-L,N-P,R,S,U,W-Y,A'-C', 50 µm.
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