spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Analysis of Ptch1 and ß-catenin mutant skeletons. (A) Skeletal preparations of 16.5 dpc mouse embryos. A forelimb of each embryo is shown in the lower panel. The posterior skull in the Ptch1c/-; Col2a1-Cre mutant did not form (arrow). Skull formation in the Ptch1c/-; Catnbc/-; Col2a1-Cre double mutant was rescued (arrow). Mineralization (arrows, lower panel) in the long bones was more severely reduced in the Ptch1c/-; Catnbc/-; Col2a1-Cre double mutant than in either of the single mutants. S, scapular; H, humerus; R, radius; U, ulna. (B) ß-catenin is not required for Ihh signaling. Consecutive sections of the developing humerus at 14.5 dpc were examined by in situ hybridization with the indicated 35S-labelled riboprobes. In both Ptch1c/-; Col2a1-Cre and Ptch1c/-; Catnbc/-; Col2a1-Cre mutant embryos, expression of the Hh signaling targets Hip1 and Gli1, and of Pthrp, was similarly upregulated in cartilage, perichondrium and joints (arrows). The perichondrium was slightly thinner in the Ptch1c/-; Catnbc/-; Col2a1-Cre mutant than in the Ptch1c/-; Col2a1-Cre mutant.





Right arrow Return to article