|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Analysis of osteoblast differentiation in ß-catenin and
Ptch1 mutant embryos. (A,B) Consecutive sections of
the developing humerus at 14.5 dpc (A) and 16.5 dpc (B) were examined by von
Kossa staining and in situ hybridization with the indicated
35S-labelled riboprobes. (A) At 14.5 dpc, ColX expression
was missing, and expression of the early osteoblast markers Runx2 and
Osx was activated in both Ptch1c/-; Col2a1-Cre
and Ptch1c/-; Catnbc/-; Col2a1-Cre mutants.
Ossification and expression of the late osteoblast markers Opn and
Mmp13 were only detected in the wild-type embryo. (B) At 16.5 dpc,
ColX expression was still missing in the Ptch1c/-;
Col2a1-Cre and Ptch1c/-; Catnbc/-;
Col2a1-Cre mutants. Osx expression was still stronger in the
Ptch1c/-; Catnbc/-; Col2a1-Cre mutant than in
the Catnbc/-; Col2a1-Cre mutant. Ossification and
expression of Opn and Mmp13 were enhanced in the
Ptch1c/-; Col2a1-Cre mutant, but diminished in
Catnbc/-; Col2a1-Cre and Ptch1c/-;
Catnbc/-; Col2a1-Cre mutants.
|
