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Fig. 3. ß-catenin activation of Myf5 expression in PSM and somite
explants. (A-F) PSM explants (A-C) and somite explants (D-F)
infected with a lentiviral vector carrying the cDNA for a stabilized form of
ß-catenin (Dp). (A,D) Hoechst staining of the nuclei. Infected cells were
visualized by GFP fluorescence (B,E), and
Myf5nlacZ/+-expressing cells were detected by
immunofluorescence with an anti-ß-galactosidase monoclonal antibody
(C,F). (G,H) Quantitative analysis of
Myf5nlacZ activation in infected PSM (G) and somite (H)
explants. Explants were infected with Dp or 
C ß-catenin
lentiviruses and co-cultured with or without 2 nM N-Shh recombinant protein
(Shh). Explants co-cultured with neural tube (NT) were used as a positive
control; explants infected with lentiviruses carrying GFP cDNA only
were used as a negative control (C). Independent experiments (n=8)
were performed in triplicate and averaged.
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