DEV02544 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Schematic drawing of C. elegans CDC-37 protein and its homologues in other species. The amino-terminal portion of the protein is the CDC-37 client-binding domain. Percent identity and similarity are noted for each.

• Supplemental Figure 2 -

  Fig. S2. GFP::CDC-37 protein is depleted by cdc-37(RNAi). Extracts from embryos expressing GFP::CDC-37 after RNAi treatment probed with anti-GFP antibody. Left lane, vector-only control; right lane, cdc-37(RNAi). Anti-tubulin was used as a loading control.

• Supplemental Figure 3 -

  Fig. S3. Depletion of CDC-37 affects the distribution of NMY-2::GFP. Each image is from a wide-field microscopy time-lapse movie of a single embryo through the first cell cycle. (A,B) Control NMY-2::GFP embryos showing NMY-2::GFP foci early in the cell cycle (A), and the break-down of the clusters in late metaphase (B). (C,D) cdc-37(RNAi) NMY-2::GFP embryos showing reduced posterior clearing, although the clusters and filaments of protein early in the cell cycle look wild type in size (C), and the lack of protein at the cortex at metaphase (D).