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Fig. 4. Cell lineage analyses of control and mutant mesoderm cells. DiI was
applied to the primitive streak mesoderm (A-F). After culture,
embryos were observed under visible light (A'-F') or
ultra violet light (A''-F''). (A-B'') DiI was
injected into the proximal region of the primitive streak (arrowheads) of
control (A) or mutant embryos (B) at the late streak stage. Arrows indicate
the embryonic/extra-embryonic border. Embryos injected with DiI were cultured
for around 30 hours (A',A',B,B', ventral view). Mesoderm
cells derived from proximal primitive streak of control embryos contributed to
lateral plate (A', arrowhead and yolk sac, A'', arrow), but not to
somites (A',A'', asterisk). In Bmpr-MORE embryos, mesoderm
cells derived from proximal primitive streak contributed to regions anterior
and lateral to paraxial region (B',B'', arrowheads and arrow,
respectively) but apparently not to the paraxial region (B',B'',
asterisks). (C-D'') DiI was injected into the anterior-to-distal region
of the primitive streak (arrowheads) of control (C) or mutant (D) embryos at
the late streak stage. Embryos injected with DiI were cultured for around 30
hours (C' and C'', dorsal view; D' and D'', ventral
view). (C',C'') Mesoderm cells derived from the anterior- to-distal
primitive streak of control embryos contributed to somites (asterisks) and to
an axial structure (red arrows and arrowheads, respectively), but not to
lateral plate (blue arrows) (see inset). (D',D'') In
Bmpr-MORE embryos, mesoderm cells derived from the anterior-to-distal
primitive streak contributed to a region anterior to somites (arrowhead) and
an axial structure (arrow). (E-F'') DiI was injected into the middle
region of the primitive streak (E,F, arrowhead) of control and mutant embryos
of the head-fold stage. Arrows indicate the length of the primitive streak.
Embryos injected with DiI were cultured for around 24 hours. (E',E'')
Dorsal views. (F',F'') Ventral view. (E',E'') In control
embryos, mesoderm cells derived from middle primitive streak contributed to
lateral plate mesoderm (arrowheads), but not to somites (asterisks).
(F',F'') In Bmpr-MORE embryos, mesoderm cells derived from
middle primitive streak mainly contributed to lateral row of somites
(arrowhead) and fewer mesoderm cells contributed to medial row of somites
(arrow). Scale bars: 100 µm for A-D; 250 µm for and E,F; 300 µm for
A-B'',D-F''; 600 µm for C',C''.
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