DEV02551 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. ES cells were differentiated in SCM alone or in the presence of (A,C) Dkk1-His or of (B,D) commercially available Dkk1 at the indicated concentrations and analyzed at days 3-5 for Flk1 expression and cell viability, as determined by forward- and side-scatter profile. (E) Total protein staining of purified Dkk1-His shows a major band of appropriate size that displays immunoreactivity with α-His antibody (F), and a minor band of higher apparent M_r consistent with the albumin carrier protein.

• Supplemental Figure 2 -

  Fig. S2. A2.sβcat ES cells were differentiated in SRM alone or in the presence of Bmp4, doxycycline or Bmp4 + doxycycline at the indicated cell densities. At day 4, cells were evaluated by FACS for viability based on forward- and side-scatter profiles.

• Supplemental Table - Microsoft Excel